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TRIpure Reagent(Trizol总RNA提取试剂)

TRIpure Reagent(Trizol总RNA提取试剂)

品 牌 aidlab
货 号 RN0102
规 格 100 ml
价 格(元) 580.00
咨询优惠价
货号 规格 价格
RN0102 100 ml 580.00
RN0101 50 ml 360.00

产品说明

  TRIpure 试剂是直接从细胞或组织中提取总RNA的试剂。它在破碎和溶解细胞时能保持RNA的完整性。加入氯仿后离心,样品分成水样层和有机层。RNA存在于水样层中。收集上面的的水样层后,RNA可以通过异丙醇沉淀来还原。在除去水样层后,样品中的DNA和蛋白也能相继以沉淀的方式还原。乙醇沉淀能析出中间层的DNA,在有机层中加入异丙醇能沉淀出蛋白。共纯化DNA对于样品间标准化RNA的产量十分有用。 
无论是人、动物、植物还是细菌组织,该方法对少量的组织(50-100mg)和细胞(5×106)以及大量的组织(≥1g)和细胞(>107)均有较好的分离效果。TRIpure 试剂操作上的简单性允许同时处理多个的样品。所有的操作可以在一小时内完成。TRIpure抽提的总RNA能够避免DNA和蛋白的污染。故而能够作RNA印迹分析、斑点杂交、poly(A)+选择、体外翻译、RNA酶保护分析和分子克隆。如果是用于PCR,当两条引物位于单一外显子内时,建议用扩增级的DNase I来处理抽提的总RNA。
TRIpure 试剂能促进不同种属不同分子量大小的多种RNA的析出。例如,从大鼠肝脏抽提的RNA琼脂糖凝胶电泳并用溴化乙啶染色,可见许多介于7 kb和15 kb之间不连续的高分子量条带(mRNA和hnRNA成分),两条优势核糖体~5 kb (28S)和~2 kb(18S),低分子量RNA介于0.1和0.3 kb之间 (tRNA, 5S)。当抽提的RNA用TE稀释时其A260/A280比值≥1.8。

适用范围:

适用于各种动植物组织/细胞总RNA、DNA、蛋白的快速抽提

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4.     Effect of Sanguisorba tannins on the expression of MGMT gene and protein in myelosuppressed mice. TUMOR Vol. 33, September 2013

5.     Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126

6.     Effects of Du Meridian Electroacupuncture on Growth Associated Protein- 43 Expression in Rats after Spinal Cord Injury. Chin J Rehabil Theory Pract, Jan. 2014, Vol. 20, No.1.

7.     Mycobacterium Bovis Ornithine Carbamoyltransferase,MB1684, Induces Proinflammatory Cytokine Gene. Expression by Activating NF-kB in Macrophages. ORIGINAL RESEARCH ARTICLE. DNA AND CELL BIOLOGY. Volume 33, Number 5, 2014ª Mary Ann Liebert, Inc.Pp. 1–9. DOI: 10.1089/dna.2013.2026.

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9.     The Response to High Magneto-Gravitational Environment of Different Differentiating-Stage Osteoblasts. Chinese Journal of Cell Biology 2013, 35(7):

10.  Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51