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Choline Oxidase

Choline Oxidase

品 牌 Sigma
C A S 号 9028-67-5
别 名 Choline: oxygen 1-oxidoreductase
货 号 C5896
规 格 100uN
价 格(元) 1364.00
咨询优惠价
货号 规格 价格
C5896 100uN 1364.00

产品说明

包装
100, 500, 1000 units in poly bottle
50 units in glass bottle
Unit Definition
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
Application
Choline oxidase from Alcaligenes sp. has been used in a study to investigate a choline biosensor constructed with chitinous membrane and its application in measuring cholinesterase inhibitory activities. Choline oxidase from Alcaligenes sp. has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.
Choline oxidase from Alcaligenes sp. has been used to evaluate choline biosensors constructed with chitinous membranes. The enzyme has also been used to analyse the photoreaction of choline oxidase with covalently bound flavin, using spectroscopic techniques. Furthermore, the product can be used for cholinesterase activity and phospholipid determination assays.[1][2]
Biochem/physiol Actions
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as the intermediate and molecular oxygen as th eprimary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the study of the reaction mechanism for the conversion of choline to the aldehyde intermediate, & of betaine-aldehyde to glycine-betaine.[3] The enzyme is a flavoprotein with a molecular weight of approx. 72,000 Da (gel filtration) or 66,000 Da (SDS gel electrophoresis).[4]
Physical properties
Isoelectric point: 4.1 ± 0.1
Michaelis constants: 2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure: One mol of FAD is covalently bound to mol of the enzyme
Inhibitors: p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH: 8.0 – 8.5
Optimum temp: 40 – 45°C
pH Stability: pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability: Below 37°C (pH 7.5, 10min)


性质:


Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Cholinesterase and Organophosphate Detection Reagents, Coupling Enzymes,
form lyophilized powder
mol wt mol wt ~95 kDa
storage temp. −20°C