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蛋白酶

蛋白酶

品 牌 Sigma
C A S 号 9036-06-0
别 名 Actinase E Pronase E
货 号 P5147
规 格 100mg
价 格(元) 559.00
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货号 规格 价格
P5147 100mg 559.00

产品说明

Frequently Asked Questions
Frequently Asked Questions are available for this Product.
Features and Benefits
• highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8
• compatible with many DNA and RNA isolation buffers
• broad substrate specificity
包装
1, 5 g in glass bottle
100 mg in glass bottle
Preparation Note
Collected from culture broth of S. griseus.
Quality
Contains calcium acetate.
Specificity
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Application
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Streptomyces griseus has been used in crystallographic and kinetic investigations of the covalent complex formed by tetrapeptide aldehydes and serine proteases[2].
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
The enzyme from Sigma has been used for the digestion and analysis of antithrombin-heparin complexes.[1] It has also been used for the isolation of enzyme-resistant starch.[3]
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease from Streptomyces griseus is a mixture of at least three proteolytic activities including an extracellular serine protease. Serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
This product is a mixture of at least three caseinolytic activities and one aminopeptidase activity.[4] The caseinolytic enzymes were named as Streptomyces griseus Protease A, Streptomyces griseus Protease B and Streptomyces griseus Trypsin. This product may be used when extensive or complete degradation of protein is required. This protease mixture is highly nonspecific and can digest casein to the extent of >70% as mono-amino acids.
Physical properties
Completely inactivated by heating above 80 °C for 15-20 minutes.
Protocols & Applications
Non-specific Protease Activity Assay Video


性质:


Related Categories Additional Reagents, Application Index, Carbohydrate-active Enzymes, Core Bioreagents, Core Bioreagents Enzymes,
type Type XIV
form powder
solubility 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble0.2 mg/mL at 37 °C, clear, colorless to tan
shipped in wet ice
storage temp. −20°C