欢迎进入昊鑫官网

电话订购 · 意见建议400-861-5108

脱氧核糖核酸酶 I DNase I

脱氧核糖核酸酶 I DNase I

品 牌 Sigma
C A S 号 9003-98-9
别 名 DNase I 脱氧核糖核酸 5′-寡核苷酸-水解酶
货 号 D5025
规 格 15KU
价 格(元) 959.00
咨询优惠价
货号 规格 价格
D5025 15KU 959.00

产品说明

Frequently Asked Questions
Frequently Asked Questions are available for this Product.
Analysis Note
由缩二脲测定的蛋白质。
Application
用于从蛋白质样品中除去 DNA。
DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes.[1] In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids.[2]
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
包装
15000 units in glass bottle
150000, 375000, 750000 units in poly bottle
Preparation Note
10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate.
Physical form
含氯化钙的冻干粉
Biochem/physiol Actions
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+.[3] The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme.[4] A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found.[7] 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)[6] and actin[5] are known to inhibit the enzyme activity.


性质:


Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Enzyme Class Index,
Quality Level PREMIUM
type Type IV
form lyophilized powder
mol wt mol wt ~31 kDa
purified by chromatography
composition Protein, ≥80%
solubility 0.15 M NaCl: soluble5.0 mg/mL, clear, colorless
Featured Industry Diagnostic Assay Manufacturing
Diagnostic Assay Manufacturing
foreign activity Chymotrypsin ≤0.5%
Protease ≤0.05%
RNase ≤0.02%
shipped in wet ice
storage temp. −20°C