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第一链反转录试剂盒

第一链反转录试剂盒

品 牌 aidlab
别 名 TRUEscript 1st Strand cDNA Synthesis Kit
货 号 PC1802
规 格 50 次x 20μl反应体系
价 格(元) 900.00
咨询优惠价
货号 规格 价格
PC1802 50 次x 20μl反应体系 900.00

产品说明

储存: -20℃ 保存,有效期12个月

制品说明:


本制品以RNA为模板,用TRUEscript Hˉ RTase /RI Mix、5×RT Reaction Mix高效合成第一链cDNA,操作简单。制品使用通过基因重组技术克隆表达的点突变型RNase H活性缺失的M-MuLV反转录酶TRUEscript Hˉ RTase。野生型的M-MuLV 包含的RNase H活性能够催化降解DNA/RNA杂合体中的RNA,因此在cDNA第一条链的合成反应中可能会降解RNA/DNA杂合体中的模板RNA。本酶M-MuLV(RNase Hˉ)的RNase H活性缺失,与M-MuLV 相比,具有更强的延伸能力和稳定性,可用于较长的cDNA合成以及高比例的全长cDNA文库的构建等。

使用该产品发表的部分文章:


1. Inhibitory Effects of MicroRNA-34a on Cell Migration and Invasion of Invasive Urothelial Bladder Carcinoma by Targeting Notch1. J Huazhong Univ Sci Technol[Med Sci], 2012, 32(3) :375-382
2. Effect and Molecular Mechanism of DHA on Improving Rats Learning and Memory Ability. Food Science, online 2012,11-9
3. NOTCH1 functions as an oncogene by regulating the PTEN/PI3K/AKT pathway in clear cell renal cell carcinoma. UROLOGIC ONCOLOGY, 2012
4. Overexpression of small heat shock protein 21 protects the Chinese oak silkworm Antheraea pernyi against thermal stress. Journal of Insect Physiology, IP 3099 No. of Pages 7, Model 5G 14 June 2013
5. Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126
6. 基质金属蛋白酶Ⅱ(mmps-2)在脑胶质细胞瘤瘤体中表达的临床研究报告. 北方药学2012年第9 卷第4期.
7. Effect of Chinese herbal drug‐containing serum of Huoxue Bushen prescription on the proliferation of human umbilical vein endothelial cells and on expression of endothelial nitric oxide synthase through p42/44MAPK pathway. Chin J Geriatr, November 2013, Vol.32 , No.11.
8. Characterization and Function of a Gene Pc 14-3-3 Isoform from Red Crayfish, Procambarus clarkii. Pakistan J. Zool., vol. 46(1), pp. 107-113, 2014.
9. Di-(2-ethylhexyl) phthalate and bisphenol A exposure impairs mouse primordial follicle assembly in vitro. First published:24 January 2014 Full publication history, DOI:10.1002/em.21847.
10. Primordial follicle assembly was regulated by notch signaling pathway in the mice. Mol Biol Rep (2014) 41:1891–1899. DOI 10.1007/s11033-014-3038-4.
11. Cloning and Expression Analysis of CsCER7, a Relative Gene May Regulate Wax Synthesis in Cucumber. Acta Horticulturae Sinica 2014,41(4): 661-671
12. Effect of KCTD15 gene on 3T3-L1 preadipocyte by global expression profile microarray. J Third mil Med Univ. Vol.36,NO.9,May15 2014
13. Stem apex detoxification culture markedly improved severalphysiological characters of chrysanthemum ‘YUTAI’. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0541-1
14. Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51
15. Overexpression of a NF-YB3 transcription factor from Picea wilsonii confers tolerance to salinity and drought stress in transformedArabidopsis thaliana. Plant Physiology and Biochemistry 2015, 94: 153-164
16. Molecular characterization of a 14-3-3 zeta gene from Plodia interpunctella: A potential marker for phylogenetic inference. Biochemical Systematics and Ecology 2015, 60: 171-176
17. Molecular cloning and expression analysis of a myosin light chain 1 (MLC-1) gene from Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae). Entomological Research 2015, 45: 305-313
18. Small Heat Shock Proteins and Eicosanoid Pathways Modulate Caspase-1 Activity in the Fat Bodies of Antheraea pernyi. first posted online September 3, 2015; doi: http://dx.doi.org/10.1101/026039