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零背景pTOPO-TA Simple克隆试剂盒/不含多克隆酶切位点MCS

零背景pTOPO-TA Simple克隆试剂盒/不含多克隆酶切位点MCS

品 牌 aidlab
别 名 Zero Background pTOPO-TA Simple Cloning Kit
货 号 CV1501
规 格 20次×10μl
价 格(元) 480.00
咨询优惠价
货号 规格 价格
CV1501 20次×10μl 480.00
CV1502 80次×10μl 1780.00

产品说明

本制品和传统的T4连接酶原理不同,它利用了Topoisomerase可以在瞬间(几秒钟-几分钟)、高效(接近100%)连接DNA片段的原理采用本公司独创的工艺制成。
1. 可以在瞬间(几秒钟-几分钟)完成连接。
2. 无需冰浴和热休克,室温5分钟内完成转化;无需1小时复苏,只需37℃10分钟复苏便可以涂板。从连接到涂板只需15-20分钟。
3. 无自连、零背景,无需繁琐蓝白斑筛选,见到长出的克隆便是有插入的(接近100%)。
4. 可以连接长达10kb片段(即使连接5kb片段,挑10个菌落,至少8个是有插入的),是目前世界上最简单、最快速、零背景免筛选的TOPO TA克隆载体。
注:本TOPO载体连接体系是按照10l计算,如果按照其它公司产品5l计算,实际使用次数可以增加一倍,20次实际可用40次。

使用该产品发表的部分文章:


CV14 Zero Background pTOPO-TA Cloning Kit:
1. Occurrence and genetic diversity of Grapevine berry inner necrosis virus from grapevines in China. Plant Disease 2016, doi: http://dx.doi.org/10.1094/PDIS-05-16-0694-RE
2. Development and Application of a Quantitative RT-PCR Approach for Quantification of Grapevine fanleaf virus. Acta Horticulturae Sinica 2016, 43 (3): 538-548.
3. An adenine nucleotide translocase (ANT) gene from Apostichopus japonicus; molecular cloning and expression analysis in response to lipopolysaccharide (LPS) challenge and thermal stress. Fish & Shellfish Immunology 2016, 49: 16–23
4. Cloning and ExpressionAnalysis of AcLOX1 Gene in Onion (Allium cepa). Journal of Agricultural Biotechnology 2016, 24(4): 548-556
5. Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China. Scientific Reports 2016, 6:36406, DOI: 10.1038/srep36406
6. Occurrence and Genetic Diversity of Grapevine berry inner necrosis virus from Grapevines in China. Plant Disease 2017, 101(1): 144-149

CV16 Zero Background pTOPO-Blunt Cloning Kit:
1. Complete nucleotide sequence of a new variant of grapevine fanleaf virus from northeastern China. Arch Virol 2016, doi:10.1007/s00705-016-3101-7

CV17 Zero Background pTOPO-Blunt Simple Cloning Kit::
1. Cloning and Expression Analysis of Citrate Synthase(CS)Gene in Camellia oleifera. Bulletin of Botanical Research 2016, 36(4): 556-564
2. Cloning and expression analysis of a autophagy-related protein 8 encoding gene in Polyporus umbellatus. Chin Med Biotechnol 2016, 11(5):415-419