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EASYspin Plus 植物RNA快速提取试剂盒

EASYspin Plus 植物RNA快速提取试剂盒

品 牌 aidlab
货 号 RN3802
规 格 50次
价 格(元) 1300.00
咨询优惠价
货号 规格 价格
RN3801 20次 650.00
RN3802 50次 1300.00

产品说明

产品介绍:

     本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶,植物RNA助提剂PLANTaid帮助结合多糖多酚并通过离心去除,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,基因组DNA清除柱子同时吸附清除残留的DNA, 然后RNA被选择性洗脱滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点:

1.完全不使用有毒的苯酚,氯仿等试剂,也不需要乙醇沉淀等步骤。 
2.简捷,单个样品操作一般可在25分钟内完成,世界上最简单快速的试剂盒。 
3.独有的植物RNA助提剂可以有效结合多糖多酚,提高清除效果。 
4.独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。 
5.适应性极其广泛,可以提取包括棉花、松针、冬青树叶、葡萄叶片、等100多种国内外试剂盒提取失败的样品。详细样品列表请参考公司主页产品介绍。 
6.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。
使用EASYspin/EASYspin Plus试剂盒已经有大量文章发表,请联系我们索取发表的文章和包括北京林业大学,北京农学院,中国农业大学,农林科学院果树研究所,林业所,中国农科院等大学和研究院所大量使用案例。

使用本产品发表的部份文章:


1.     桃果实、花、根、叶:Isolation, characterisation and phylogenetic analysis of resistance gene    analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science,    2011, 91(6): 961-970

2.     樱桃花、叶、颚等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium    L.)    causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December    2012

3.     洋葱根、茎、蕾、叶、雌雄蕊等各部位:Cloning and Expression Analysis of A Putative B Class MADS-box    Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769

4.     芜菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid       Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January    2012,    Pages 47–59

5.     芜  菁 1 :EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H

6.     番茄叶:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science  Bulletin 2012,28(10):132-135

7.     栀子叶:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural    Science Bulletin.2012, 28(27):194-198

8.     油桐果实:Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong.     Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP)  from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August

9.     油桐果实1:Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time    PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084

10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra    haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35(12):1814-1821

11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709

12. 石斛1:ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705

13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012

14. 大豆1:Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012

15. 红花玉兰:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5

16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104

17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online:15 March, 2013

18. :五倍子1:Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9

19. 青杄 :cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401

20. 青杄 1:cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206

21. 青杄 2:Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49,No. 10, Oct. 2013.

22. 洋葱:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y

23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17.

24. 木瓜1:Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C:

25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x

26. 柑橘1:Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 1–8.

27. 柑橘2: Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.

28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.

29. 栀子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198

30. 丹参:Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.

31. 牡丹:Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996

32. 东南景天:Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165.

33. 山苍子:Identification of appropriate reference genes for normalizing transcript expression by quantitative real‑time PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1

34. 木本植物:Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0

35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.

36. 桃子:Biochemical changes and defence responses during the development of peach gummosis caused by Lasiodiplodia theobromae. Eur J Plant Pathol (2014) 138:195–207, DOI 10.1007/s10658-013-0322-4.

37. 桃子1:Carbohydrate metabolism changes in Prunus persica gummosis infected with Lasiodiplodia theobromae. Phytopathology "First Look" paper • http://dx.doi.org/10.1094/PHYTO-01-13-0025-R • posted 11/27/2013.

38. 海棠:The Malus crabapple transcription factor McMYB10 regulatesanthocyanin biosynthesis during petal coloration. Scientia Horticulturae 166 (2014) 42–49.

39. 海藻:A rapid and sensitive method for field detection of Prorocentrum donghaiense using reverse transcription-coupled loop-mediated isothermal amplification. Harmful Algae 29 (2013) 31–39.

40. 油茶:Establish a cDNA-AFLP Technology System in Camellia oleifera. Molecular Plant Breeding, 2013, Vol.11, No.5, 611-616.

41. 亚洲百合:Transcriptomic analysis of Asiatic lily in the process of vernalization via RNA-seq. Mol Biol Rep. DOI 10.1007/s11033-014-3250-2.

42. 毛泡桐:Dynamic expression of novel and conserved microRNAs and their targets in diploid and tetraploid of Paulownia tomentosa. Biochimie xxx (2014) 1e10.

43. 人参:Cloning and Sequence Analysis Squalene Epoxidase Gene in Panax gin-seng. Journal of Jilin Agricultural University 2014, 36(2): 149-152,17

44. 雪莲:Cloning and Sequence Analysis of rbcs Gene from Sasussured involucrdta Kar. et Kir. Chinese Agricultural Science Bulletin 2014, 30(15): 261-267

45. 柑橘3:Secreted Expression of Cecropin B Gene Enhances Resistance to Xanthomonas axonopodis pv. citri in Transgenic Citrus sinensis‘Tarocco’ Acta Horticulturae Sinica 2014, 41(3): 417–428 http: // www. ahs. ac. cn

46. 菊花:Stem apex detoxification culture markedly improved severalphysiological characters of chrysanthemum ‘YUTAI’. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0541-1

47. 荞麦和拟南芥:Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant. Gene 2014, 550(2): 200–206

48. 油松:Differential expression of SLOW WALKER2 homologue in ovules of female sterile mutant and fertile clone of Pinus tabulaeformis. Russian Journal of Developmental Biology 2014, 45(2): 78-84

49. 玫瑰花:Precise spatio-temporal modulation of ACC synthase by MPK6 cascade mediates the response of rose flowers to rehydration. The Plant Journal 2014, 79(6): 941–950

50. 棉花和拟南芥:Functional characterization of GhAKT1, a novel Shaker-like K+ channel gene involved in K+ uptake from cotton (Gossypium hirsutum). Gene 2014, 545(1): 61–71

51. 棉花和拟南芥1:Upland Cotton Gene GhFPF1 Confers Promotion of Flowering Time and Shade-Avoidance Responses in Arabidopsis thaliana. PLoS ONE 2014, 9(3): e91869. doi:10.1371/journal.pone.0091869

52. 白杨:Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0536-y

53. 毛果杨:Molecular characterization of the SPL gene family in Populus trichocarpa. BMC Plant Biology 2014, 14: 131

54. 葛根:Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata. Plant Cell Reports 2014, 33(7), 1173–1185

55. 百合:Cloning and Expression Analysis of Actin Gene(lilyActin)from Lily. Acta Horticulturae Sinica 2013, 40(7): 1318–1326

56. 百合1: Vernalization of Oriental hybrid lily ‘Sorbonne’: changes in physiology metabolic activity and molecular mechanism. Molecular Biology Reports 2014, DOI 10.1007/s11033-014-3545-3

57. 黄鹌菜:Transcriptome Sequencing and De Novo Analysis of Youngia japonica Using the Illumina Platform. PLoS ONE 2014, 9(3): e90636. doi:10.1371/journal.pone.0090636

58. 棉花1:Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers. PLoS ONE 2014, 9(5): e96537. doi:10.1371/journal.pone.0096537

59. 苹果:Low Medium pH Value Enhances Anthocyanin Accumulation in Malus Crabapple Leaves. PLoS ONE 2014, 9(6): e97904. doi:10.1371/journal.pone.0097904


其它公司多糖多酚植物RNA提取试剂盒失败原因和解决方案

很多植物RNA的样品由于含有大量的多糖、多酚、代谢产物、色素等成分,造成RNA提取过程中氧化、褐化、降解、由于植物品种的多样性造成情况更加复杂。手工的CTAB类的方法提取因为时间太长,太繁琐,手工方法不在讨论之列。一直以来没有一款好的试剂盒包括qiagenpromega等进口试剂盒也无法满足科研工作者对植物RNA提取的要求。

部分成功样品:

植物:棉花、海棠、黑加仑、烟草、拟南芥、虎杖、大豆、草莓、冬青、月季花雌蕊、蔷薇、沙棘、冬枣、芦荟、仙人掌、报春花、水稻、玉米、唐菖蒲、樱桃、白玉兰、毛白杨、樱花、葡萄、百合花、百合叶子雌蕊雄蕊、紫菜、绿藻、香蕉、水仙花、青花菜、地被菊、苹果、梅花、番茄、石斛、毛桃、苎麻、慈姑、葛根、甘肃桃、玫瑰花、槟榔果、甜糖菊、硅藻、牡丹、胡杨、油桐果、梨子皮、板栗花序、青皮云杉、红树根、铁线蕨、黄瓜、小麦叶子种子、番木瓜、甘薯、紫薯、油松、油茶、马尾松、芜菁、毛果杨、木薯、大叶落地生根、山杏、旱柳、桉树、琵琶花果、丹参、人参、西洋参、栀子、洋葱、红豆杉、梨树叶、五倍子、泡桐、西瓜、芍药、雪莲等等,其中包括qiagen无法提取的黑加仑、冬青、月季、松针、葡萄叶片等,promega无法提取的海棠等样品、均可用该产品成功提取。

真菌:桃褐腐病菌(Monilinia fructicola)、菇类等。

推荐:本公司的EASYspin 植物RNA提取试剂盒为独家产品,世界领先水平,不使用苯酚,氯仿有毒性物质,采用离心柱型操作,添加有自主创新的多糖多酚去除成分,是多糖多酚样品的特效产品,20分钟内得到高质量RNA。成功提取Promega无法提取的海棠叶片、Qiagen无法提取的黑加仑。是国产试剂赶超世界先进水平的一个典型例子。